R WANG 1, J ZHANG 2, G LIU 2, L HAO 2, Y WANG 1, D HARRIS 1, D WANG 2, C QI 1
1Centre For Transplant And Renal Research, Westmead Institute For Medical Research, The University Of Sydney, WESTMEAD, Australia, 2Department of Nephrology, The Second Hospital of Anhui Medical University, HEFEI, CHINA
Background: Innate lymphoid cells (ILCs) are a newly identified heterogeneous family of innate immune cells with critical roles in immunity, tissue homeostasis, and pathologic inflammation. We conducted this study to investigate the frequency of circulating ILC subsets and analyse the relationship between ILCs and clinical parameters in various chronic kidney diseases (CKD).
Methods: We analysed 93 peripheral blood samples from patients with CKD, including diabetic nephropathy (DN), lupus nephritis (LN), IgA nephropathy (IgAN), membranous nephropathy (MN). Flow cytometry was used to examine the total ILCs, ILC1s, ILC2s, and ILC3s. Pearson correlation for a parametric measure or Spearman correlation for a nonparametric measure was used to observe the correlation between proportion of ILCs and clinical parameters.
Results: In DN, the proportion of total ILCs and ILC subgroups increased significantly. Positive correlations between proportion of total ILCs, ILC1s and body mass index (BMI) were observed in DN. The proportion of ILC2 was also positively correlated with BMI and triglyceride (TG). In LN, a significantly increased proportion of ILC1 was found in parallel with a reduced proportion of ILC2s. The proportions of total ILCs and ILC1 were correlated with the level of GFR and C3, and ILC3 had a positive correlation with C3 and platelet count. In all enrolled patients, the proportion of total ILCs and ILC1s was significantly correlated with the concentration of ACR and the level of GFR and BUN.
Conclusion: The proportion of circulating ILC subgroups increased significantly in various types CKD and correlated with various clinic-pathological features. Although the meaning of these correlations is uncertain, they reflect a possible role for ILCs in these conditions.
Dr Wang is an associate chief physician in the Department of Nephrology, the Second Affiliated Hospital of Anhui Medical University. She is focus on the application of immune cells in the chronic kidney disease.