PATIENT-DERIVED ENDOTHELIAL CELL FUNCTION IS PREDICTED BY CONTROLLED BLOOD PRESSURE

HUUSKES BM1, KERR PG2, SAMUEL CS3, RICARDO SD1

1Biomedical Discovery Institute, Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria; 2Department of Medicine, Monash Medical Center and Monash University, Clayton, Victoria; 3Biomedical Discovery Institute, Department of Pharmacology, Monash University, Clayton, Victoria

Aim: To determine if clinical parameters observed in patients on haemodialysis affected the appearance of patient-derived endothelial cells in culture.

Background: Low circulating endothelial progenitor cell (EPC) numbers have been linked to worsening outcomes for dialysis patients. EPC function can be measured by their ability to form colony forming units (CFU) and late outgrowth endothelial cells (OECs) in vitro. Numerous clinical parameters may affect EPC function, yet their affect on OEC appearance remains unknown.

Methods: Dialysis-dependent patients (n=20) were recruited to this study; and their age, time on dialysis, blood pressure (BP), EPO, statin use and smoking status was collected. Blood (10mls) was obtained prior to a single dialysis session and peripheral blood mononuclear cells isolated and cultured. After 7 days CFU was assessed, and cells were further cultured for 21 days or until OECs appeared (identified by cobblestone morphology). Correlations between patient clinical parameters and OEC appearance were investigated.

Results: Patient age, time on dialysis, statin use, and BP did not correlated with circulating EPC level. However, patients administered EPO had lower circulating EPCs compared to patients who were not (p<0.05). Only 9/20 patient-derived cells formed OECs, which was not correlated to starting %EPC or any other clinical parameter except for BP. OECs were significantly greater in patients who had low systolic (p<0.01) and diastolic (p<0.05) BP.

Conclusions: BP affects dialysis-dependent patient-derived OEC numbers in culture, suggesting that controlling BP may be key to maintaining vascular health in patients on dialysis.

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